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It contains a high concentration.
Msa plate no growth. Psuedomonas aeruginosa (Gram negative) - no growth. Mannitol Salt Agar (MSA) is used to determine if the bacteria is halophilic (salt loving) and if the bacteria can ferment mannitol. Mannitol Salt Agar (MSA) is used as a selective and differential medium for the isolation and identification of Staphylococcus aureus from clinical and non-clinical specimens.
It encourages the growth of a group of certain bacteria while inhibiting the growth of others. Left plate growing. Mannitol Salt Agar (MSA) Plate.
Staphylococcus aureus (Gram positive) - growth with yellow halo. MSA helps to demonstrate the ability of a bacterium to grow in a 7.5% salt environment (growth indicates tolerance for high salt environment – no growth means intolerance). Mannitol salt agar or MSA is a commonly used selective and differential growth medium in microbiology.
A growth medium is considered differential if, when specific microbes are present, the medium, or bacterial colonies themselves, exhibit a. This medium is important in medical laboratories as one method of distinguishing pathogenic microbes in a short period of time. It is recommended for the detection and enumeration of coagulase-positive Staphylococci in milk, food and other specimens and encourages the growth of a group of certain bacteria while inhibiting the growth of others.
Mannitol Salt Agar contains peptones and beef extract, which supply nitrogen, vitamins, minerals and amino acids essential for growth. The 7.5% concentration of sodium chloride results in the partial or complete inhibition of bacterial organisms other than staphylococci. Usually, Staphylococci have the ability to tolerate salt concentration.
Staphylococcus aureus and Staphylococcus epidermidis on mannitol salt agar Mannitol salt agar is a commonly used growth medium in microbiology. Staphylococcus epidermidis (Gram positive) - growth but no yellow halo. The primary purpose of mannitol salt agar is to determine or select bacteria that have the ability to grow in a high salt environment.
It encourages the growth of a group of certain bacteria while inhibiting the growth of others. It contains a high concentration of salt, making it selective for most Gram-negative and some Gram-positive bacteria since this level of salt is inhibitory to most other bact. If there is no growth after adding salt, it means that the microorganism is intolerant to salt.
Escherichia coli (Gram negative) - No growth.
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